Catalog Number - 59350-01-RP

MicroBee Amino™ is a traditional aminopropyl HPLC stationary phase developed for laboratories requiring a proven alternative to legacy amino column methods. As part of the Cogent™ Similars product line, it has been widely used for many years in carbohydrate analysis, ion-exchange related separations, and other applications where amino selectivity is desired. The stationary phase consists of a bonded amino functional group attached to high-purity Type-A silica, providing retention characteristics comparable to traditional amino columns used in established methods.

59350-01-RP is a 300 mm x 3.9 mm ID analytical column packed with 10 µm, 125Å MicroBee Amino particles. This dimension closely matches the format used in many historical HPLC methods developed on Microbondapak™ Amino and similar columns, making it particularly useful for method transfer and replacement applications. The 300 mm length provides maximum resolving power and increased theoretical plate counts for challenging separations, while the 3.9 mm ID format typically performs well at flow rates of approximately 0.8–1.5 mL/min on conventional HPLC systems.

This USP L8 column is supplied packed and shipped in reversed phase solvents, making it convenient for laboratories operating reversed phase amino methods without solvent exchange prior to installation. It serves as an excellent alternative for methods developed on Microbondapak™ Amino and similar stationary phases while providing the unique selectivity of an aminopropyl bonded phase. The extended 300 mm length is especially useful when additional resolution is required for complex sample matrices or closely eluting compounds.

Key Benefits

• Traditional aminopropyl stationary phase

• Suitable alternative for USP L8 methods

• Compatible with many legacy Microbondapak™ methods

• Packed and shipped in reversed phase solvents

• Maximum resolution from 300 mm column length

• Unique amino phase selectivity

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

Typical Applications

• USP L8 methods

• Reversed phase HPLC

• Method transfer projects

• Pharmaceutical analysis

• Complex sample separations

• Quality control testing

• Carbohydrate analysis

• Legacy amino column replacement

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Amino

• USP Classification: L8

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: Aminopropyl

• Endcapped: No

• Pore Size: 125Å

• Column Dimensions: 3.9 mm ID x 300 mm

• Length: 300 mm

• Recommended Flow Rate: 0.8–1.5 mL/min

• Shipping Solvent: Reversed Phase

• Brand: Cogent

Important Notes

• Packed and shipped in reversed phase solvents

• USP L8 column classification

• Ideal for legacy method replacement

• Compatible with conventional HPLC systems

• Unique aminopropyl selectivity

• Extended column length provides maximum resolving power

• Alternative to Microbondapak™ Amino columns

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